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- Herbarium genomics: skimming and plastomics from archival specimens: Webbia: Vol 72, No 1
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Oxidative stress in the brain: novel cellular targets that govern survival during neurodegenerative disease. Prog Neurobiol. Biol Signals Recept.
International Journal of Diabetes Research. Concerted dysregulation of 5 major classes of blood leukocyte genes in diabetic ZDF rats: A working translational profile of comorbid rheumatoid arthritis progression. International Journal of Prevention and Treatment. Med Sci Monit Basic Res. The biology of social attachments: sopiates alleviate separation distress. Biol Psychiatry.
Herbarium genomics: skimming and plastomics from archival specimens: Webbia: Vol 72, No 1
Pierce RC, Kumaresan V. The mesolimbic dopamine system: The final common pathway for the reinforcing effect of drugs of abuse? Neurosci Biobehav Rev. Schmauss C, Emrich HM. Dopamine and the action of opiates: a reevaluation of the dopamine hypothesis of schizophrenia. With special consideration of the role of endogenous opioids in the pathogenesis of schizophrenia. Assessment of the relationship between lipid parameters and obesity indices in non-diabetic obese patients: a preliminary report. J Biol Chem. Culture volume and vessel affect long-term survival, mutation frequency, and oxidative stress of Escherichia coli.
Appl Environ Microbiol. Resveratrol protects vascular smooth muscle cells against high glucose-induced oxidative stress and cell proliferation in vitro. Sodium nitrite provides angiogenic and proliferative effects in vivo and in vitro. Davila AF, Zamorano P. Mitochondria and the evolutionary roots of cancer. Phys Biol. Chemolithoheterotrophy in a metazoan tissue: thiosulfate production matches ATP demand in ciliated mussel gills. J Exp Biol.
Heat flux, oxygen flux, and mitochondrial redox state as a function of oxygen availability and ciliary activity in excised gills of Mytilus edulis. J Exp Zool. J Pineal Res. Crawling leaves: photosynthesis in sacoglossan sea slugs. J Exp Bot. Photophysiology of kleptoplasts: photosynthetic use of light by chloroplasts living in animal cells.
Plastid survival in the cytosol of animal cells. Trends Plant Sci. Pennisi E. Modern symbionts inside cells mimic organelle evolution. Intracellular invasion of green algae in a salamander host. Kao WC, Hunte C. The molecular evolution of the Qo motif.
Genom Biol Evol. Tomato fruit chromoplasts behave as respiratory bioenergetic organelles during ripening. Plant Physiol. Oxygen sensing and signaling. Annu Rev Plant Biol. Kleine T, Leister D. Emerging functions of mammalian and plant mTERFs. Biochim Biophys Acta. Massively convergent evolution for ribosomal protein gene content in plastid and mitochondrial genomes.
Genome Biol Evol. Evolutionary defined role of the mitochondrial DNA in fertility, disease and ageing. Hum Reprod Update. A genomic timescale for the origin of eukaryotes. BMC Evol Biol. Mitochondria: Major regulators of neural development. Stefano GB. Conformational matching: a possible evolutionary force in the evolvement of signal systems. In: Stefano GB, editor. CRC Handbook of comparative opioid and related neuropeptide mechanisms. Cost and safety are important factors for applications as positive selection herbicides to control weeds in transgenic crops and negative selection agents to control the spread of transgenic crops.
A dual selectable marker gene has not previously been described in plastids.
The bacterial codA gene, encoding cytosine deaminase Mullen et al. Transplastomic cells expressing codA are sensitive to the antibiotic 5-fluorocytosine Serino and Maliga, , which is used to treat fungal infections in humans, with possible side effects including hepatotoxicity Steer et al. The relatively high cost of 5-fluorocytosine and its potential toxicity on nontarget organisms would hinder its use as a negative selection agent to manage the spread of transgenic crops expressing codA. Plastids are important centers for amino acid synthesis and metabolism Lancien et al.
Here, we have developed a plastid marker based on the Schizosaccharomyces pombe dao gene Wood et al. Our results are consistent with d -amino acids being transported into plastids, where d -Ala inhibits one or more plastid functions and d -Val is converted into a toxic product by DAAO. The plastid dao gene provides a new versatile marker for plastid genetics, with applications for developing d -Ala-tolerant transplastomic plants, d -Val-based containment of transgenic plastids, and controlling the timing of recombination events in plastid genomes.
A dao gene was identified in the nuclear genome of the fission yeast S. The S. The amino acid S. The prominent kD band was not visible in lanes corresponding to pET30 cultures vector without dao gene or uninduced pET dao cultures Fig. The predicted size of the amino acid S.
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Cell extracts containing the overexpressed S. Expression of the S. Coomassie blue-stained total soluble protein was from E. MW, Molecular weight marker; nt, not tested. The dao expression cassette was combined with the plastid aadA marker gene and inserted between the rbcL and accD genes in a previously cloned 7. The resulting Map of tobacco plastid transformation vector pUM dao The vector targets the integration of aadA and dao genes to the Aoc I site located at base 59, of the ,bp tobacco plastid genome Yukawa et al.
The Aoc I site is located in the intergenic region between the rbcL and accD data not shown genes.
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Shown are tobacco plastid-targeting arms, aadA , dao , native rbcL genes, and the vector pAT Twigg and Sherratt, Total leaf DNA from four plastid transformants pt dao lines 1—4 isolated from independent transformation events was analyzed by DNA-blot analysis. The rbcL gene is located on an An rbcL hybridization probe showed replacement of the This confirms targeted integration and homoplasmy of transgenic plastid genomes in the four pt dao transplastomic lines studied.
A probe containing only dao coding sequences hybridized to the predicted 0.
Targeted integration and homoplasmy of the plastid dao marker gene. Tobacco regulatory elements were used unless indicated as follows: B. Hin dIII H sites and hybridization probes are located. Shown are wild-type and independent transplastomic pt dao lines, molecular weight MW standards, and band sizes. Expression of the dao gene in two independent lines lines 1 and 2 was studied by RNA-blot analysis.
Blots containing leaf RNA from wild-type and transplastomic leaves fractionated on denaturing formaldehyde gels Fig. The rbcL probe hybridized to a single 1.